After the gst fusion proteins were processed by factor xa and were purified by gluta - thione - sepharose 4b affinity chromatography , the recombinant protein hbrp with high purification would be acquired . during the process of separation and purification , the every result which can show the location of the aim protein was monitored by 10 % sds - page . the gst fusion proteins acquired finally were examined and analyzed by the methods of western - blottin 原核表達(dá)系統(tǒng)具有表達(dá)效率高,操作方便等特點,但缺乏翻譯? 3 ?后修飾,以pgex載體表達(dá)的gst hbrp融合蛋白,具有表達(dá)的蛋白穩(wěn)定性好,在體外易于純化等優(yōu)點,表達(dá)的gst融合蛋白可用gluthioneseph刪sc4b進(jìn)一步純化一factorxa酶切與測分離,從而大量獲得hbrp重組蛋白,而且一次回收率大約在90以上。
factor xa meaning:[Medicine] Activated form of factor X that participates in both the intrinsic and extrinsic pathways of blood coagulation. It catalyzes the conversion of prothrombin to thrombin in conjunction with ot...